The principle of the microplate reader - Huaqiang Electronic Network

24V, 17pF, surface mount CAN bus ESD protection diode

Photocoupler

Microplate reader

working principle:

The microplate reader is actually a phase-change photoelectric colorimeter or spectrophotometer. The basic working principle is basically the same as that of the main structure and photoelectric colorimeter. The illustration is a working principle of a single-channel automatic injection microplate reader. The light wave emitted by the light source lamp becomes a bundle of monochromatic light through a filter or a monochromator, and enters the sample to be tested in the microporous pole of the plastic. Part of the monochromatic light is absorbed by the specimen, and the other part is irradiated through the specimen. On the photodetector, the photodetector converts the different optical signals with different strengths and weaknesses into corresponding electrical signals. The electrical signals are preamplified, logarithmic amplification, analog-to-digital conversion and other signals are processed and sent to the micro-processing. The data is processed and calculated, and finally the results are displayed by the display and the printer. The microprocessor also controls the movement of the mechanical drive mechanism in the X and Y directions by the control circuit to move the microplate to achieve the automatic injection detection process. The microplate reader uses a manual moving microplate for inspection, thus eliminating the mechanical drive mechanism and control circuit in the X and Y directions, which makes the instrument smaller and simpler in structure.

The microplate is a transparent plastic plate specially packaged for placing the sample to be tested. The plate has a plurality of rows of small holes of uniform size, and the corresponding antigen or antibody is embedded in the hole. A small hole can hold a few milliliters of solution.

Light is an electromagnetic wave, and a wavelength of 100 nm to 400 nm is called ultraviolet light, and a light between 400 nm and 780 nm can be observed by the human eye, and more than 780 nm is called infrared light. People can see the color because the light is reflected on the object and reflected back by the object. Green plants are green because most of the plants absorb red orange and blue-violet light, but they do not absorb and reflect green, so the plants appear green. The principle of the microplate reader is to detect the absorbance of the analyte at a specific wavelength.

With the development of detection methods, the single-chip microplate reader with multiple detection modes is called multi-function microplate reader, which can detect absorbance (Abs), fluorescence intensity (FI), time-resolved fluorescence (TRF), fluorescence polarization (FP). ), and chemiluminescence (Lum).

The microplate reader can be divided into a grating type microplate reader and a filter type microplate reader in principle. The grating type microplate reader can intercept any wavelength in the wavelength range of the light source, and the filter type microplate reader can only detect a specific wavelength according to the optional filter.

Shanghai Bayu (

In 2007, Industrial Co., Ltd. set up an offline supply system for laboratory instruments, MRO industrial products, etc. In 2009, it officially launched an online procurement operation platform. As the earliest enterprise engaged in MRO industrial products and laboratory instruments and meters in Shanghai, Shanghai Bayu Industrial Co., Ltd. has strong strength, scientific management, quality assurance, complete after-sales and reputation first.